Riders will leave Kenora and will hit Dryden, Ignace and finish off in Sioux Lookout with a dinner and awards ceremony
Executive Director Lynn Moffatt encourages the public to come out and support the cause.
Moffatt says they are hoping to raise $40,000 and all money raised supports 1,000 people living with the disease in the Kenora and Rainy River District.
Riders will also participate in a poker derby.