The OPP say one lane of the Trans-Canada has been opened following a fatal multiple vehicle collision about 30 kilometers east of Kenora.
Police say a westbound transport jack-knifed near Dogtooth Lake around 1:00 this morning.
An eastbound semi was unable to avoid the truck and collided with it.
Police say the driver of the eastbound unit was killed.
A short time later another tractor-trailor unit and a passenger vehicle also slid into the first two trucks.
There was one other accident reported last night, when a transport rolled over near Chadwick Lake on Highway 17 east and lost its load.
The OPP say the driver was not injured in that mishap.