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The OPP say an ATV went off the side of the highway near the Airport Road Saturday night around 10:30 and drove into a guard rail.
Both the driver and the passenger were taken to Lake of the Woods District Hospital for treatment.
The passenger sustained only minor injuries and was released, while the driver was later transferred to the Health Sciences Center in Winnipeg with serious injuries.
The OPP's Technical Traffic Collision Unit is helping out with out with the investigation.